Abstract
The Staphylococcus aureus Recombinant plasmids pS194 and pSC194, which confer resistance to streptomycin and streptomycin-chloramphenicol, respectively, have been used as vectors for the construction of recombinant DNA, since each of them carries a unique receptor site for the EcoRI endonuclease.
The hybrid DNA does not express resistance to streptomycin, a marker that is present in both vectors, presumably because the marker gene is cut by EcoRI. A chloramphenicol marker present on pSC194 was used for positive hybrid selection. Hybrid plasmids generated by ligating pSC194 with one or more of the four EcoRI fragments of the large (18.1·106 daltons) staphylococcal plasmid p1258 were constructed and allowed us to develop a physical map to p1258.
Product name
Recombinant protein Staphylococcus aureus Staphylococcus Enterotoxin G (His tag)
Purity: > 90% SDS-PAGE.
Expression system: Escherichia coli
Accession: P0A0L7
Protein length: full length protein
Animal-free: No
Nature: recombinant
Species: staphylococcus aureus
Predicted Molecular Weight: 43 kDa including tags
Amino acids: 26 to 258
Tags: Your N-Terminus Tag
Additional sequence information
Strain N315. This product is the full-length mature protein from aa 26 to 258 with a 6xHis-SUMO tag at the N-terminus. The signal peptide is not included.
Specifications
Our Abpromise warranty covers the use of ab225609 in the following tested applications. Application notes include recommended starting dilutions; the end-user must determine the optimal dilutions/concentrations.
Applications: SDS PAGE
Form: Liquid
Concentration: 100 µg to 0.5 mg/mL
Stability and Storage
- Shipped at 4°C. At the time of aliquot delivery. Store at -20°C or -80°C. Avoid freeze/thaw cycle.
- Components: 50% glycerol (glycerin, glycerin), Tris buffer