Cameron Kelley

Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.

Bacillus coagulans has good potential as an industrial manufacturing organism for platform chemical compounds from renewable assets however has restricted genetic instruments out there. Here, we current a focused gene disruption system utilizing the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression. Enormously quick RNA hydrolysis …

Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans. Read More »

Microfluidic impedance spectroscopy as a tool for quantitative biology and biotechnology.

Microfluidic impedance spectroscopy as a tool for quantitative biology and biotechnology.

A microfluidic gadget that is ready to carry out dielectric spectroscopy is developed. The gadget consists of a measurement chamber that’s 250 μm thick and 750 μm in radius. Around 1000 cells match contained in the chamber assuming common portions for cell radius and quantity fraction. This quantity is about 1000 folds decrease than the …

Microfluidic impedance spectroscopy as a tool for quantitative biology and biotechnology. Read More »

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology.

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology.

Cyanobacteria are appropriate for sustainable, solar-powered biotechnological purposes. Synthetic biology connects biology with computational design and an engineering perspective, however requires environment friendly tools and details about the operate of organic elements and techniques. To allow the event of cyanobacterial Synthetic Biology, a number of molecular tools had been developed and characterised: (i) a broad-host-range …

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Read More »

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